Nexera Bio is a biocompatible UHPLC system offering the same
superior reliability, robustness, and expandability as our flagship Nexera
UHPLC systems. But, Nexera Bio is distinctive in that it is well suited to the
analysis of protein-based pharmaceuticals, antibody drugs, and other substances
related to biotechnologies, genetic engineering, cell fusion, and cell
culturing.
Nexera Bio is compatible with mobile phases containing high
concentrations of salts or acids typically used for analyzing antibody drugs
and membrane proteins. Its low surface activity inhibits peak tailing
experienced on traditional UHPLC systems. Such tailing can be especially
problematic when analyzing phosphorylated peptides, which have a high affinity
for metals.
Nexera Bio Features
1. Low Carryover
Shimadzu autosamplers have a reputation for the lowest
carryover in the industry. Nexera Bio features specially selected materials and
a rinsing mechanism that further inhibit carryover from biological samples.
Peak detection at low levels and chromatographic reproducibility are
dramatically improved.
2. Minimized Sample
Loss due to Adsorption
Some biological molecules adsorb readily to stainless steel
tubing. Successful assays for such samples require a system with low surface
activity to minimize this problem. The low surface activity of Nexera Bio
results in minimized sample loss and greatly improved sensitivity.
3. Improved Corrosion
Resistance
The analyses of biological samples frequently require mobile
phases with high salt concentrations, halogenated compounds or ion pairing
agents. These conditions can be problematic for stainless steel UHPLC systems.
With Nexera Bio, crucial metal-free components define the wetted surfaces while
maintaining a high-efficiency flow path (66 MPa). The system is unaffected by
high salt content (KH2PO4 in H3PO4), and the corrosion resistant design
provides trouble-free operation.
2. Nexera Bio
Advantages
2-1. High-Sensitivity
Analysis
Instrument Sensitivity Comparison for ATP Analysis
Because ATP exhibits strong affinity for metals, analysis
using a general-purpose UHPLC is difficult because of analyte binding in the
system. By contrast, Nexera Bio inhibits such adsorption, making good peak
shapes and high sensitivity a reality.
2-2. Excellent
Reproducibility
The figure to the right shows a comparison of peak area
values from 10 successive ATP analyses (standard solution at 1 mg/L) using
Nexera Bio and general-purpose UHPLC systems.
Because of its low surface activity, Nexera Bio achieves much
higher sensitivity and better reproducibility than the general-purpose UHPLC
system.
3. Consumables Ideal
for the Analysis of Biopharmaceuticals
Shim-pack Bio Diol and IEX columns offer performance that can
help increase the accuracy of analyzing and evaluating biopharmaceuticals and
drugs based on mid-sized molecules.
3-1. Example Using
Shim-pack Bio Diol Columns for Evaluating Aggregates in Antibody Drugs
In this example, Shim-pack Bio Diol water-based size
exclusion chromatography columns were used to evaluate aggregates in an
antibody drug.
Available in four different pore sizes, Shim-pack Bio Diol
columns are suitable for size exclusion chromatography and intended for
bioanalysis. They are ideal for analyzing aggregates or separating fragments in
antibody drugs, separating nucleic acids or sugar chains, and for measuring
molecular weight.
The figure below shows an example using a Shim-pack Bio Diol
column to analyze an antibody drug.
Reducing the particle size from 5 µm to 2 µm improved aggregate
separation and also resulted in higher sensitivity and quantitative accuracy.
Furthermore, high-speed analysis was achieved by using a shorter (150 mm long)
column.
3-2. Example Using a
Shim-pack Bio IEX Column for Synthetic Oligonucleotides
The Shim-pack Bio IEX is an ion-exchange column suitable for
separation of proteins and nucleic acids. Shim-pack Bio IEX columns are
available with either non-porous or porous particles and are intended for
laboratory-scale purification of small quantities.
For the figure below, a non-porous Shim-pack Bio IEX column
was used to analyze synthetic oligonucleotides. The Shim-pack Bio IEX is able
to distinguish between molecules that differ by only one salt base.
3-3. Example of Peptide
Analysis Using a Mastro™ C18 Metal-Free Column
The chromatogram shown below was obtained by successive
analysis of a standard solution of angiotensin I (100 mg/L; n=5) using a Mastro
C18 high-pressure metal-free column in a Nexera Bio system.
The 0.12 % relative standard deviation (n = 5) in peak area
values indicates excellent reproducibility.
Nexera Bio system combined with a Mastro C18 column achieved
both low adsorption and high sensitivity, which should help improve the
reliability of biopharmaceutical analysis.
4. Column Product Line
4-1. Shim-pack Bio Diol
Ordering Information
4-2. Shim-pack Bio IEX
4-3. Mastro C18 High-Pressure
Metal-Free Column
Peak tailing due to system adsorption is common when
analyzing compounds containing a phosphate group, such as phosphorylated
peptides and nucleotides. Mastro™ series columns are high-pressure, metal-free
columns that feature a polymer-lined stainless steel column body and highly
pressure-resistant polymeric frit. Two models are available in the Mastro
series, the Mastro™ C18 and the Mastro™ PFP2 (pentafluorophenyl).
The Mastro™ PFP2 (pentafluorophenyl) is a reversed-phase
column suitable for separating compounds that are difficult to retain on many
C18 columns. Examples are positional isomers and some halogenated compounds.
The figure to the right compares results obtained from a
standard solution of ATP (10 mg/L) in a Nexera Bio system using a
general-purpose ODS column (stainless steel column) and a Mastro C18
high-pressure metal-free column.
The ATP could not be detected using the general-purpose ODS
column, due to adsorption to the metal surfaces inside the column, but the ATP
was detected without any sample loss using the polymer-lined Mastro C18. These
results show how the Nexera Bio system in combination with a Mastro C18 column
can be used to reliably analyze nucleotides and other compounds that include a
phosphate group.
5. Nexlock PLS
High-Pressure PEEK Tubing and Finger-Tightened Fittings
Nexera Bio systems use highly pressure-resistant PEEK tubing
(Nexlock PLS*), reinforced with stainless steel, to connect the autosampler and
column. The fittings seal the tubing connection in such a way that minimizes
dead volume helps to prevent peak tailing. Because they are finger-tight, no
tools are needed.
* 66 MPa pressure capacity indicated in specifications, with
replacement recommended after 100 uses
6. TORAST H-Bio Vial
Reportedly, the main mechanisms for adsorption of peptides to
vials are hydrophobic adsorption and ionic adsorption. With glass vials, ionic
adsorption to silanol groups at the glass surface dominates. With polymeric
vials, hydrophobic adsorption dominates. Shimadzu GLC has developed a polymeric
vial with a special coating that minimizes the adsorption of peptides. Results
have indicated that the vial is inert to basic, acidic, and neutral compounds.
It is especially recommended for LC and LC/MS(/MS) users that frequently
analyze low-concentration samples.
ĐẠI DIỆN ĐỘC QUYỀN SHIMADZU TẠI VIỆT NAM
Hotline: 0902 550 957
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