On-Column
Derivatization GC-MS System
Operation Procedure:How
On-Column Derivatization Works with a Two-Step Auto Injector
The sample to be
analyzed is automatically derivatized using the following procedure.
The MBTFA (N-Methylbis-trifluoroacetamide) derivatizating
agent and sample solution to be analyzed are taken up into the syringe.
The sample is injected, vaporized in the injection port and
introduced onto the head of the capillary column.
After the components to be analyzed are introduced into the
capillary column, the MBTFA
derivatizating agent is injected.
The components to be analyzed are derivatized in the
capillary column, and the derivatives undergo GC/MS analysis.
Application
Example:Analysis of Urine Sample by On-Column Derivatization GC-MS
The test sample was obtained by treating a urine sample as
shown in the figure below. TFA derivatives of methamphetamine (MA) and its
metabolite amphetamine (AP) were detected from urine sample #1. With urine
sample #2, MDMA and TFA derivatives of its metabolites MDA and HMMA, as well as
TFA derivatives of trace amounts of methamphetamine and amphetamine, were
detected. By using this technique, the process of derivatization has been
simplified and analysis efficiency improved; moreover, amphetamine and
methamphetamine and its analog components can be detected at very high sensitivity.
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